《植物生理学报》 2018, 54(3): 421-428

钠盐和氯化物对真盐生植物盐地碱蓬营养生长的影响

李艳迪, 郭建荣, 王宝山^*

山东师范大学生命科学学院, 逆境植物重点实验室, 济南250014

通信作者：王宝山；E-mail: bswang@sdnu.edu.cn

摘　要：

200 mmol·L^-1 NaCl显著促进真盐生植物盐地碱蓬(Suaeda salsa)的营养生长, 但具体是Na⁺、Cl^-还是二者共同起作用尚不清楚。本文分别采用0、200 mmol·L^-1 NaCl以及相同浓度的钠盐(由NaNO₃、NaH₂PO₄、Na₂SO₄组成)、氯化物(由MgCl₂、KCl、CaCl₂、NH₄Cl组成)自种子播种开始处理盐地碱蓬, 对植株的生物量、离子含量、光合和荧光等营养生长相关参数进行研究, 结果表明: 200 mmol·L^-1 NaCl和钠盐处理显著增加盐地碱蓬的株高、分枝数、干鲜重等, 其中200 mmol·L^-1钠盐处理的促进作用最显著; 而200 mmol·L^-1氯化物处理显著抑制了盐地碱蓬的营养生长, 植物矮小, 黄化严重。进一步研究发现NaCl和钠盐处理显著增加了盐地碱蓬叶片中叶绿素含量, 提高了植株净光合速率; 而同浓度氯化物处理降低了盐地碱蓬叶片中叶绿素含量, 其净光合速率显著下降。以上结果表明: Na⁺通过促进光合作用促进稀盐型盐生植物盐地碱蓬的营养生长, 而Cl^-有抑制作用。

关键词：盐地碱蓬; 盐胁迫; 钠盐; 氯化物; 营养生长

收稿：2017-10-09   修定：2018-01-10

资助：国家自然科学基金(31570251)、山东省自然科学基金(ZR2017MC003)和山东省高校科技计划项目(J17KA136)。

Effects of sodium salt and chloride on vegetative growth of euhalophyte Suaeda salsa

LI Yan-Di, GUO Jian-Rong, WANG Bao-Shan^*

Key Laboratory of Plant Stress, College of Life Science, Shandong Normal University, Jinan 250014, China

Corresponding author: WANG Bao-Shan; E-mail: bswang@sdnu.edu.cn

Abstract:

NaCl (200 mmol·L^-1) significantly promotes the vegetative growth of euhalophyte Suaeda salsa. However, the single effects of sodium and chloride are still unclear. To investigate the roles of sodium and chloride on the vegetative growth of S. salsa, plant biomass, ion content, chlorophyll content, photosynthesis and fluorescence parameters were determined under the conditions of control, 200 mmol·L^-1 NaCl, 200 mmol·L^-1 sodium salt (composed of NaNO₃, NaH₂PO₄ and Na₂SO₄) and 200 mmol·L^-1 chloride (composed of MgCl₂, KCl, CaCl₂ and NH₄Cl), respectively. The results show that the plant height, fresh weight, dry weight and branch number of S. salsa were significantly promoted by NaCl and sodium salt treatments, while inhibited by chloride treatment. Further experiments indicate that the content of chlorophyll, net photosynthetic rate, Φ_PSII and F_v/F_m of S. salsa were also markedly increased when treated by NaCl and sodium salt, and significantly decreased by 200 mmol·L^-1 chloride treatment. The results indicate that Na⁺ was the main factor to promote the vegetative growth of S. salsa via improving the photosynthesis parameters.

Key words: Suaeda salsa; saline stress; sodium salt; chloride; vegetative growth